The aim of this project is to exam the proteome of the gram-positive pathogens Enterococcus faecium and Staphylococcus aureus in order to identify novel targets for antibiotic resistance treatment. To achieve this goal wildtype antibiotic susceptible strains of Enterococcus faecium and Staphylococcus aureus are transformed with plasmids that contain Antibiotic Resistance Genes (ARG) which confer resistance to several important antibiotics, such as methicillin, vancomycin and chloramphenicol. The proteomes of these transformant and the antibiotic susceptible wild types are examined via Liquid chromatography–mass spectrometry (LC-MS) and notable differences between the transformed and untransformed strains will be further examined. This poster presentation outlines a novel protocol that was developed for this project in order to transform the antibiotic susceptible strain Enterococcus faecium NCTC13169 with a pUCIDT-goldengate plasmid that had been constructed to contain the gene cfrA, which confers chloramphenicol resistance.