Immersive technologies allow the integration of virtual content and the physical environment, currently explored for higher education as well as professional activities (e.g. smart manufacturing). Users are invited to perceive and interact with multimodal interfaces, using multiple senses, such as visual, auditory, touch, as well as motion and speech. When defining requirements to design immersive technologies such as Virtual Reality, Augmented Reality and 3D interaction, it is a challenge to assess measures for users’ performance and engagement. First, a review of existing technologies such as VR headsets, depth cameras, stereoscopic displays and spatial audio systems will identify gaps that need to be addressed on future studies for a better accessibility and usability of interactive systems. Then, the analysis of the literature will be used to design experimental protocols and user studies assessing how users perceive different issues such as delays in systems’ input and output (e.g. motion-to-photon latency). Expected results will have an impact on how immersive technologies are designed, allowing personalisation to different user needs, according to the context of use.